Therapy for corneal diseases, layer by layer

Osteotropic therapy via targeted layer-by-layer nanoparticles.

Current treatment options for debilitating bone diseases such as osteosarcoma, osteoporosis, and bone metastatic cancer are suboptimal and have low efficacy. New treatment options for these pathologies require targeted therapy that maximizes exposure to the diseased tissue and minimizes off-target side effects. This work investigates an approach for generating functional and targeted drug carri...

Protein and Lactose Separation by Modified Ultrafiltration Membrane using Layer by Layer Technique

Layer-by-Layer (LbL) is a method which can be used for nanoscale coating and surface functionalization of a material. LbL technique mainly uses the electrostatic attracting between charged materials (polyelectrolytes, nanoparticles, etc.) and an oppositely charged surface. In this study, protein separation (BSA) from lactose solution was carried out using the LbL self-assembly method, which was...

Use of Alloplant Biomaterial in Layer-by-layer Barrier Keratoplasty of Vascular Corneal Leukoma

Layer-by-layer desquamation of corneal epithelium and maturation of tear-facing membranes.

A method to devitalize single layers of apically exposed rabbit corneal epithelial cells through the use of digitonin is described. Devitalized cells exfoliate spontaneously as loosely cohesive, trypan-blue-stained layers, exposing underlying viable cells. Repeated application of this devitalization-exfoliation methodology results in the gradual elimination of each of the epithelial cells. The ...

Fabrication of Poly(ε-Caprolactone), Hydrophilic and β-Tricalcium Phosphate Layer- by -Layer Nanofibrous Scaffolds for Tissue Engineering

In this study, using biodegradable polymers, nanofiberouse scaffolds were fabricated from the layer-by-layer electrospinning method, including two layer that poly (ε-caprolactone), polyvinylpyrrolidone deposited at first layer and poly (ε-caprolactone), polyvinyl alcohol , β-tricalcium phosphate at latter. After prepration of scaffolds, scanning electron microscopy (SEM), swelling, porosity, me...